Mouse Growth Regulated Oncogene Beta (GROb) ELISA Kit

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SKU:
MODL00586
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P10889
Sensitivity:
6.0pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
CXCL2,SCYB2,MIP2,GRO2,MIP2a,MGSAb,CINC2a,HSF,Macrophage inflammatory protein 2-alpha,Hematopoietic synergistic factor,Chemokine(C-X-C-Motif)ligand 2
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
Frequently bought together:

Description

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Mouse Growth Regulated Oncogene Beta (GROb) ELISA Kit

The Mouse Growth Regulated Oncogene Beta (GRO-β) ELISA Kit is specially designed for the quantitative detection of GRO-β levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.GRO-β is a key chemokine protein that plays a crucial role in inflammation and immune response, promoting cell migration and activation.

This protein is implicated in various physiological and pathological processes, including cancer, inflammatory diseases, and autoimmune disorders. The GRO-β ELISA Kit provides researchers with a valuable tool for studying the functions of this protein and its potential implications in disease development and therapeutic interventions.

Product Name:Mouse Growth Regulated Oncogene Beta (GROb) ELISA Kit
Product Code:MODL00586
Size:96 Assays
Target:GROb
Synonyms:CXCL2, SCYB2, MIP2, GRO2, MIP2a, MGSAb, CINC2a, HSF, Macrophage inflammatory protein 2-alpha, Hematopoietic synergistic factor, Chemokine(C-X-C-Motif)ligand 2
Detection Method:Sandwich
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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