Mouse Growth Differentiation Factor 11 (GDF11) ELISA Kit

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SKU:
MODL00537
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9Z1W4
Sensitivity:
1.39pg/mL
Range:
3.125-200pg/mL
ELISA Type:
Sandwich
Synonyms:
BMP11,Bone morphogenetic protein 11
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
Frequently bought together:

Description

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Mouse Growth Differentiation Factor 11 (GDF11) ELISA Kit

The Mouse Growth Differentiation Factor 11 (GDF11) ELISA Kit is a highly sensitive and specific assay designed for the precise measurement of GDF11 levels in mouse serum, plasma, and tissue lysate samples. This kit provides accurate and reproducible results, making it suitable for a variety of research applications.GDF11 is a key regulator of developmental processes, playing a crucial role in growth and differentiation.

It is involved in various biological functions, including tissue regeneration and aging, making it a valuable target for studying age-related diseases and regenerative medicine.With its advanced technology and reliable performance, the Mouse GDF11 ELISA Kit is essential for researchers interested in investigating the role of GDF11 in different biological processes and exploring potential therapeutic interventions.

Product Name:Mouse Growth Differentiation Factor 11 (GDF11) ELISA Kit
Product Code:MODL00537
Size:96 Assays
Target:GDF11
Synonyms:BMP11, Bone morphogenetic protein 11
Detection Method:Sandwich
Range:3.125-200pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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