The Mouse Gro (Growth Regulated Oncogene) Alpha/CXCL1 ELISA Kit is specifically designed to accurately detect levels of Gro Alpha/CXCL1 in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Gro Alpha/CXCL1 is a key chemokine involved in inflammation, immune response, and cell migration. It plays a crucial role in various physiological and pathological processes, making it a valuable biomarker for studying inflammatory diseases, cancer, and immune disorders.
With its reliable performance and user-friendly protocols, the Mouse Gro Alpha/CXCL1 ELISA Kit is an indispensable tool for researchers seeking to investigate the role of Gro Alpha/CXCL1 in disease progression and development of therapeutic interventions.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GROα/CXCL1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GROα/CXCL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GROα/CXCL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse GROα/CXCL1. You can calculate the concentration of Mouse GROα/CXCL1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
95-108
93-108
Average (%)
98
101
98
1:4
Range (%)
100-112
80-92
94-108
Average (%)
106
87
101
1:8
Range (%)
100-115
86-100
92-109
Average (%)
107
92
99
1:16
Range (%)
99-115
86-98
93-106
Average (%)
107
91
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-101
93
EDTA plasma (n=5)
89-102
95
Cell culture media (n=5)
94-106
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.1
153.4
344.9
55.0
140.7
312.6
Standard deviation
2.8
8.3
16.2
3.5
5.9
10.0
C V (%)
5.37
5.41
4.7
6.36
4.19
3.2
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse GROα/CXCL1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GROα/CXCL1 in samples. No significant cross-reactivity or interference between Mouse GROα/CXCL1 and analogues was observed.
