The Mouse GRN (Granulin) ELISA Kit is specifically designed for the precise measurement of granulin levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and consistent results, making it perfect for a variety of research purposes.Granulin is a key protein that plays a vital role in various physiological processes, including cell growth, inflammation, and tissue repair. Dysregulation of granulin expression has been linked to multiple diseases, such as cancer, Alzheimer's, and inflammatory disorders, making it a valuable biomarker for studying these conditions and developing potential treatments.
Overall, the Mouse GRN ELISA Kit from Assay Genie provides researchers with a powerful tool to investigate the role of granulin in health and disease, offering reliable data that can contribute to advancing scientific knowledge and therapeutic strategies.
Product Name:
Mouse GRN (Granulin) ELISA Kit
SKU:
MOES01100
Target:
Mouse GRN (Granulin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GRN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GRN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GRN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse GRN. You can calculate the concentration of Mouse GRN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-102
91-108
89-101
Average (%)
93
98
94
1:4
Range (%)
89-105
86-100
89-102
Average (%)
96
93
95
1:8
Range (%)
92-103
84-94
87-101
Average (%)
98
89
94
1:16
Range (%)
92-106
80-93
86-98
Average (%)
100
87
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-98
93
EDTA plasma (n=5)
90-103
97
Cell culture media (n=5)
84-98
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.57
9.33
47.17
4.77
10.18
48.22
Standard deviation
0.24
0.46
2.02
0.26
0.5
2.5
C V (%)
5.25
4.93
4.28
5.45
4.91
5.18
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse GRN concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GRN in samples. No significant cross-reactivity or interference between Mouse GRN and analogues was observed.
