The Mouse GPC3 (Glypican-3) CLIA Kit is a reliable and sensitive assay designed for the accurate detection of GPC3 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high specificity, ensuring precise and reproducible results for a variety of research applications.GPC3, also known as Glypican-3, is a cell surface proteoglycan that plays a crucial role in cell growth regulation and proliferation. Abnormalities in GPC3 expression have been linked to various diseases, including liver cancer and developmental disorders.
As a valuable biomarker, measuring GPC3 levels can provide insights into disease mechanisms and potential therapeutic targets.With its high sensitivity and ease of use, the Mouse GPC3 CLIA Kit is a valuable tool for studying GPC3 biology and its implications in disease pathology. Its accurate quantification of GPC3 levels makes it an essential tool for researchers in the fields of oncology, developmental biology, and biomarker discovery.
Product Name:
Mouse GPC3 (Glypican 3) CLIA Kit
SKU:
MOES00194
Target:
Mouse GPC3 (Glypican 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GPC3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GPC3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GPC3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GPC3. You can calculate the concentration of Mouse GPC3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-108
96-110
89-100
Average (%)
99
103
94
1:4
Range (%)
88-102
104-117
92-107
Average (%)
95
110
99
1:8
Range (%)
86-96
98-110
103-120
Average (%)
91
104
110
1:16
Range (%)
93-106
94-108
98-112
Average (%)
99
102
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-105
100
EDTA plasma (n=5)
96-109
103
Cell culture media (n=5)
90-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.06
209.3
692.87
87.44
205.01
662.34
Standard deviation
12.47
20.76
67.83
11.31
19.54
69.02
C V (%)
12.85
9.92
9.79
12.93
9.53
10.42
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GPC3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GPC3 in samples. No significant cross-reactivity or interference between Mouse GPC3 and analogues was observed.
