The Mouse GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) ELISA Kit is a reliable and accurate tool for the detection of GM-CSF levels in mouse serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides consistent and reproducible results, making it suitable for a variety of research applications.GM-CSF is a key cytokine involved in the regulation of immune cell development and function, playing a role in inflammatory responses, immune system function, and hematopoiesis. Its dysregulation has been linked to various diseases, including autoimmune disorders, inflammatory conditions, and cancer.
Therefore, the Mouse GM-CSF ELISA kit is an essential tool for studying these conditions and exploring potential therapeutic interventions.Overall, the Mouse GM-CSF ELISA Kit from Assay Genie is a valuable resource for researchers seeking to investigate the role of GM-CSF in health and disease, providing accurate and reliable data for further exploration and understanding in the field of immunology and beyond.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GM-CSF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GM-CSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GM-CSF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse GM-CSF. You can calculate the concentration of Mouse GM-CSF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-102
90-104
88-104
Average (%)
96
97
95
1:4
Range (%)
87-99
79-93
88-99
Average (%)
93
85
94
1:8
Range (%)
91-105
84-96
85-97
Average (%)
97
90
92
1:16
Range (%)
92-102
82-96
83-93
Average (%)
97
89
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
101
EDTA plasma (n=5)
85-99
91
Cell culture media (n=5)
93-108
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.77
95.17
346.82
46.75
93.81
328.61
Standard deviation
2.64
5.61
17.41
3.02
4.58
17.88
C V (%)
5.53
5.89
5.02
6.46
4.88
5.44
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse GM-CSF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GM-CSF in samples. No significant cross-reactivity or interference between Mouse GM-CSF and analogues was observed.
