Mouse Glutamate Receptor, Ionotropic, AMPA 4 (GRIA4) ELISA Kit

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SKU:
MODL00581
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9Z2W8
Sensitivity:
0.073ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
GLUR4,GLUR4C,GLURD,AMPA-selective glutamate receptor 4
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
Frequently bought together:

Description

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Mouse Glutamate Receptor, Ionotropic, AMPA 4 (GRIA4) ELISA Kit

The Mouse Glutamate Receptor Ionotropic AMPA 4 (GRIA4) ELISA Kit is a powerful tool for accurately measuring GRIA4 levels in mouse samples. This kit is highly sensitive and specific, providing researchers with reliable and reproducible results for a variety of research applications.GRIA4 is a key component of the AMPA receptors in the brain, playing a critical role in excitatory neurotransmission. Dysregulation of GRIA4 has been linked to neurological disorders such as epilepsy, Alzheimer's disease, and schizophrenia, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.

With its advanced technology and user-friendly design, the Mouse GRIA4 ELISA Kit is an essential resource for researchers investigating the role of GRIA4 in brain function and disease.

Product Name:Mouse Glutamate Receptor, Ionotropic, AMPA 4 (GRIA4) ELISA Kit
Product Code:MODL00581
Size:96 Assays
Target:GRIA4
Synonyms:GLUR4, GLUR4C, GLURD, AMPA-selective glutamate receptor 4
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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