Mouse Glutamate Cysteine Ligase, Catalytic (GCLC) ELISA Kit

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SKU:
MODL00533
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P97494
Sensitivity:
0.118ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
GCS,GLCL,GLCLC,Gamma-glutamylcysteine synthetase,Gamma-ECS
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
Frequently bought together:

Description

system_update_altDatasheet

Mouse Glutamate Cysteine Ligase, Catalytic (GCLC) ELISA Kit

The Mouse Glutamate-Cysteine Ligase Catalytic (GCLC) ELISA Kit is specially designed for the accurate measurement of GCLC levels in mouse samples such as serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring precise and reproducible results, making it an ideal tool for various research applications.GCLC is an essential enzyme involved in the synthesis of glutathione, a vital antioxidant and detoxifier in cells.

Dysregulation of GCLC has been linked to oxidative stress, inflammation, and various diseases including cancer, neurodegenerative disorders, and metabolic disorders. The GCLC ELISA kit provides researchers with a reliable method to study GCLC levels in mouse models, allowing for a better understanding of its role in disease pathogenesis and potential therapeutic interventions.

Product Name:Mouse Glutamate Cysteine Ligase, Catalytic (GCLC) ELISA Kit
Product Code:MODL00533
Size:96 Assays
Target:GCLC
Synonyms:GCS, GLCL, GLCLC, Gamma-glutamylcysteine synthetase, Gamma-ECS
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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