The Mouse GLUT4 (Glucose Transporter 4) CLIA Kit is a powerful tool for the quantitative detection of GLUT4 levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results for a variety of research applications.GLUT4 is a key player in glucose metabolism, facilitating the uptake of glucose into cells and maintaining blood glucose levels.
Dysregulation of GLUT4 has been implicated in conditions such as diabetes, obesity, and metabolic syndrome, making it a valuable biomarker for studying these diseases and exploring potential therapeutic interventions.Overall, the Mouse GLUT4 CLIA Kit is a valuable tool for researchers studying glucose metabolism and related diseases in mouse models, offering precise and reliable measurements for their experimental needs.
Product Name:
Mouse GLUT4 (Glucose Transporter 4) CLIA Kit
SKU:
MOES00296
Target:
Mouse GLUT4 (Glucose Transporter 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GLUT4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GLUT4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GLUT4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GLUT4. You can calculate the concentration of Mouse GLUT4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-118
89-100
89-101
Average (%)
109
94
95
1:4
Range (%)
96-110
92-104
96-112
Average (%)
103
97
103
1:8
Range (%)
85-97
100-117
87-103
Average (%)
90
107
94
1:16
Range (%)
96-110
89-101
99-114
Average (%)
102
95
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-102
93
EDTA plasma (n=5)
96-112
103
Cell culture media (n=5)
90-105
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
108.36
310.94
957.92
97.78
306.79
1005.42
Standard deviation
12.91
22.36
93.68
11.79
24.21
103.06
C V (%)
11.91
7.19
9.78
12.06
7.89
10.25
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GLUT4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GLUT4 in samples. No significant cross-reactivity or interference between Mouse GLUT4 and analogues was observed.
