The Mouse GH (Growth Hormone) ELISA Kit is specifically designed for the precise measurement of growth hormone levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and consistent results, making it an ideal tool for various research applications.Growth hormone is a key hormone that plays a vital role in regulating growth, metabolism, and other physiological processes in mice. It is essential for normal development and body maintenance.
Monitoring growth hormone levels can provide valuable insights into growth disorders, aging, and metabolic conditions, making it a valuable biomarker for studying these conditions and exploring potential therapeutic strategies.With the Mouse GH ELISA Kit, researchers can confidently analyze growth hormone levels in mouse samples with precision and efficiency, advancing their understanding of growth regulation and related pathologies.
Product Name:
Mouse GH (Growth Hormone) ELISA Kit
SKU:
MOES00672
Target:
Mouse GH (Growth Hormone)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GH. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse GH. You can calculate the concentration of Mouse GH in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-106
84-96
88-103
Average (%)
98
91
95
1:4
Range (%)
95-109
82-95
94-109
Average (%)
102
87
100
1:8
Range (%)
100-113
84-96
92-105
Average (%)
105
90
100
1:16
Range (%)
100-117
82-93
94-109
Average (%)
106
88
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-101
94
EDTA plasma (n=5)
92-104
99
Cell culture media (n=5)
90-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1
2.2
7.5
1.0
2.1
7.1
Standard deviation
0.1
0.1
0.3
0.1
0.1
0.3
C V (%)
10
4.55
4.0
10.0
4.76
4.23
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse GH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GH in samples. No significant cross-reactivity or interference between Mouse GH and analogues was observed.
