Glial cell line derived neurotrophic factor (GDNF) Family Receptor Alpha 1 (GFRA1) is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. GFRA1 is a potent survival factor for central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are its binding ligand which are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GDNF promotes the formation of a physical complex between GFRA/GDNFRa and the orphan tyrosin kinase receptor Ret, thereby inducing its tyrosine phosphorylation. The RET is a receptor tyrosine kinase representing the signal-transducing molecule of a multisubunit surface receptor complex for the GDNF, in which GFRA / GDNFRa acts as the ligand-binding component. GDNF, a distantly related member of the transforming growth factor-β (TGF-â) superfamily, and its receptor components: GFRA1, Ret and neural cell adhesion molecule (NCAM) have been recently reported to be expressed in the testis and to be involved in the proliferation regulation of immature Sertoli cells.
Product Name:
Mouse GFRA1/GDNFRA Recombinant Protein (RPES4226)
Product Code:
RPES4226
Size:
50µg
Species:
Mouse
Expressed Host:
HEK293 Cells
Synonyms:
AU042498
Accession:
AAB86600.1
Sequence:
Met 1-Ser 425
Fusion tag:
C-His
Activity:
1. Measured in a cell proliferation assay using SH?SY5Y human neuroblastoma cells. The ED50 for this effect is typically 0.3-1.5 µg/mL in the presence of 40 ng/mL Recombinant Human GDNF.2. Measured by its ability to bind with human GDNF in a functional ELISA.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain (Met 1-Ser 425) of mouse GFR?1 isoform beta (AAB86600.1) was expressed with a C-terminal polyhistidine tag.
Purity:
> 98 % as determined by SDS-PAGE
Mol Mass:
46.3 kDa
AP Mol Mass:
55-60 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
