The Mouse GDF9 (Growth Differentiation Factor 9) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of GDF9 levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for a variety of research applications.GDF9 is a key regulator of ovarian development and function, playing a critical role in folliculogenesis and oocyte maturation. Dysregulation of GDF9 has been associated with various reproductive disorders, including infertility and ovarian dysfunction.
The Mouse GDF9 CLIA Kit is therefore essential for studying these conditions and exploring potential therapeutic interventions.Overall, the Mouse GDF9 CLIA Kit from Assay Genie offers researchers a powerful and reliable tool for investigating the physiological functions and pathological implications of GDF9 in mouse models.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GDF9. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GDF9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GDF9, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GDF9. You can calculate the concentration of Mouse GDF9 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
99-113
94-108
Average (%)
100
104
101
1:4
Range (%)
86-99
93-106
97-111
Average (%)
91
99
103
1:8
Range (%)
90-103
93-109
92-107
Average (%)
95
100
99
1:16
Range (%)
99-113
95-109
86-96
Average (%)
106
102
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
100
EDTA plasma (n=5)
88-102
94
Cell culture media (n=5)
91-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
110.96
268.37
769.62
106.35
250.67
815.22
Standard deviation
11.26
28.88
50.41
13.73
24.19
75.82
C V (%)
10.15
10.76
6.55
12.91
9.65
9.3
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GDF9 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GDF9 in samples. No significant cross-reactivity or interference between Mouse GDF9 and analogues was observed.
