The Mouse GDF7 (Growth Differentiation Factor 7) CLIA Kit is a highly sensitive and specific assay designed for the precise measurement of GDF7 levels in mouse serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it an excellent choice for a variety of research applications.GDF7 is a key growth factor involved in skeletal development and the formation of bones and joints. It plays a critical role in regulating cell differentiation and bone formation, making it a valuable biomarker for studying skeletal disorders, such as osteoarthritis and osteoporosis.
With its advanced technology and easy-to-use protocols, the Mouse GDF7 CLIA Kit is a powerful tool for researchers looking to study the role of GDF7 in skeletal development and disease. Order yours today and unlock new insights into the complex mechanisms underlying bone growth and regeneration.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GDF7. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GDF7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GDF7, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GDF7. You can calculate the concentration of Mouse GDF7 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-108
89-102
90-104
Average (%)
99
94
97
1:4
Range (%)
95-111
90-104
98-113
Average (%)
101
97
105
1:8
Range (%)
100-115
88-104
88-100
Average (%)
108
95
93
1:16
Range (%)
93-106
85-98
96-112
Average (%)
99
91
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-97
92
EDTA plasma (n=5)
90-103
97
Cell culture media (n=5)
95-109
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.29
79.02
236.31
22.54
78.37
245.92
Standard deviation
2.14
7.13
21.43
2.03
7.52
21.57
C V (%)
8.81
9.02
9.07
9.01
9.6
8.77
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GDF7 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GDF7 in samples. No significant cross-reactivity or interference between Mouse GDF7 and analogues was observed.
