Mouse Gas6 (Growth Arrest Specific Protein 6) ELISA Kit
The Mouse GAS6 (Growth Arrest-Specific Protein 6) ELISA Kit is specifically designed for the precise measurement of GAS6 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and accurate results for a variety of research applications.GAS6 is a key protein involved in cell growth, survival, and proliferation, playing a critical role in various biological processes. It is particularly important in regulating immune responses, inflammation, and cell signaling pathways.
Studying GAS6 levels can provide valuable insights into the pathogenesis of diseases such as cancer, autoimmune disorders, and cardiovascular diseases.With its reliable performance and ease of use, the Mouse GAS6 ELISA Kit is an essential tool for scientists and researchers investigating the role of GAS6 in health and disease. Order your kit today and discover new possibilities for your research projects.
Product Name:
Mouse Gas6 (Growth Arrest Specific Protein 6) ELISA Kit
SKU:
MOES00850
Target:
Mouse Gas6 (Growth Arrest Specific Protein 6)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse Gas6. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Gas6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Gas6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse Gas6. You can calculate the concentration of Mouse Gas6 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-105
89-104
90-101
Average (%)
99
95
95
1:4
Range (%)
90-105
85-96
83-96
Average (%)
96
90
88
1:8
Range (%)
94-108
84-95
89-101
Average (%)
100
89
94
1:16
Range (%)
91-107
84-100
87-97
Average (%)
99
91
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-107
97
EDTA plasma (n=5)
85-97
90
Cell culture media (n=5)
92-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
268.48
428.25
1968.48
265.23
431.54
2115.57
Standard deviation
18.63
18.33
90.75
13.61
18.0
100.7
C V (%)
6.94
4.28
4.61
5.13
4.17
4.76
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse Gas6 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse Gas6 in samples. No significant cross-reactivity or interference between Mouse Gas6 and analogues was observed.
