Mouse Gas6 (Growth Arrest Specific Protein 6) CLIA Kit
The Mouse GAS6 (Growth Arrest Specific Protein 6) CLIA Kit is a reliable and efficient tool for measuring GAS6 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.GAS6 is a key protein that plays a critical role in various physiological processes, including cell growth, survival, and migration. It is also involved in immune response modulation and regulation of inflammation.
Dysregulation of GAS6 has been implicated in a range of diseases, such as cancer, cardiovascular disorders, and autoimmune conditions, making it a valuable biomarker for studying these diseases and exploring potential therapeutic interventions.Overall, the Mouse GAS6 CLIA Kit provides researchers with a valuable tool for studying the role of GAS6 in health and disease, offering reliable and actionable data for further research and clinical applications.
Product Name:
Mouse Gas6 (Growth Arrest Specific Protein 6) CLIA Kit
SKU:
MOES00171
Target:
Mouse Gas6 (Growth Arrest Specific Protein 6)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse Gas6. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse Gas6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse Gas6, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse Gas6. You can calculate the concentration of Mouse Gas6 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-98
99-112
90-101
Average (%)
92
106
95
1:4
Range (%)
93-103
97-113
85-95
Average (%)
98
104
90
1:8
Range (%)
84-96
91-106
95-108
Average (%)
90
97
100
1:16
Range (%)
95-111
100-117
100-116
Average (%)
102
107
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
100
EDTA plasma (n=5)
86-98
91
Cell culture media (n=5)
89-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
46.83
128.3
499.11
42.52
137.0
535.43
Standard deviation
4.76
12.33
56.95
4.93
10.23
48.08
C V (%)
10.16
9.61
11.41
11.59
7.47
8.98
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse Gas6 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse Gas6 in samples. No significant cross-reactivity or interference between Mouse Gas6 and analogues was observed.
