The Mouse Gal8 (Galectin-8) CLIA Kit is a powerful tool for the precise measurement of Galectin-8 levels in mouse serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research needs.Galectin-8 is a key protein involved in various cellular processes, including cell adhesion, migration, and signaling. Its dysregulation has been linked to numerous diseases, including cancer, inflammation, and autoimmune disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic approaches.
Overall, the Mouse Gal8 CLIA Kit offers researchers a reliable and efficient way to investigate the role of Galectin-8 in health and disease, paving the way for new insights and advancements in biomedical research.
Product Name:
Mouse GAL8 (Galectin 8) CLIA Kit
SKU:
MOES00280
Target:
Mouse GAL8 (Galectin 8)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GAL8. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GAL8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GAL8, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GAL8. You can calculate the concentration of Mouse GAL8 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-96
98-115
91-104
Average (%)
90
105
97
1:4
Range (%)
101-120
99-114
91-106
Average (%)
110
107
99
1:8
Range (%)
96-108
95-111
86-99
Average (%)
102
101
93
1:16
Range (%)
102-116
86-97
85-96
Average (%)
108
92
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
97
EDTA plasma (n=5)
98-113
104
Cell culture media (n=5)
87-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
108.2
225.26
774.46
115.91
231.2
836.22
Standard deviation
10.77
26.22
57.54
12.47
23.42
59.2
C V (%)
9.95
11.64
7.43
10.76
10.13
7.08
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GAL8 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GAL8 in samples. No significant cross-reactivity or interference between Mouse GAL8 and analogues was observed.
