The Mouse GAL2 (Galectin-2) CLIA Kit is a powerful tool for the precise quantification of Galectin-2 levels in mouse serum, plasma, and tissue homogenates. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.Galectin-2 is a key protein involved in various biological processes, including inflammation, immune response, and tissue repair. Its dysregulation has been linked to multiple diseases such as cancer, autoimmune disorders, and cardiovascular conditions, making it a valuable biomarker for understanding disease mechanisms and exploring therapeutic interventions.
With its advanced technology and user-friendly protocol, the Mouse GAL2 CLIA Kit from Assay Genie is an indispensable tool for researchers seeking to unravel the complexities of Galectin-2 biology and its implications in health and disease. Order yours today and take your research to the next level.
Product Name:
Mouse GAL2 (Galectin 2) CLIA Kit
SKU:
MOES00275
Target:
Mouse GAL2 (Galectin 2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse GAL2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GAL2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GAL2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse GAL2. You can calculate the concentration of Mouse GAL2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
91-103
101-115
Average (%)
92
97
106
1:4
Range (%)
91-104
97-112
85-99
Average (%)
97
105
92
1:8
Range (%)
92-106
100-118
97-109
Average (%)
98
108
103
1:16
Range (%)
90-103
98-118
90-104
Average (%)
96
107
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-96
90
EDTA plasma (n=5)
90-104
96
Cell culture media (n=5)
88-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.21
70.15
241.21
24.2
73.14
249.42
Standard deviation
2.73
6.84
21.47
2.63
7.23
19.55
C V (%)
11.28
9.75
8.9
10.87
9.89
7.84
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse GAL2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse GAL2 in samples. No significant cross-reactivity or interference between Mouse GAL2 and analogues was observed.
