Mouse G CSF Recombinant Protein (RPPB0321)
- SKU:
- RPPB0321
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Mouse
- Target:
- G CSF
- Synonyms:
- CSF3
- MGI-1G
- GM-CSF beta
- Pluripoietin
- Source:
- Escherichia Coli
- Uniprot:
- P09920
Description
| Product Name: | Mouse G CSF Recombinant Protein |
| Product Code: | RPPB0321 |
| Size: | 10µg |
| Species: | Mouse |
| Target: | G CSF |
| Synonyms: | CSF3, MGI-1G, GM-CSF beta, Pluripoietin, G-CSF, GCSF. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | G-CSF Lyophilized from 10mM NaCitrate, pH 4.0 and 150mM NaCl. |
| Solubility: | It is recommended to reconstitute the lyophilized Granulocyte Colony Stimulating Factor in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Granulocyte Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GCSF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Amino Acid Sequence: | VPLVTVSAL PPSLPLPRSF LLKSLEQVRK IQASGSVLLE QLCATYKLCH PEELVLLGHS LGIPKASLSG CSSQALQQTQ CLSQLHSGLC LYQGLLQALS GISPALAPTL DLLQLDVANF ATTIWQQMEN LGVAPTVQPT QSAMPAFTSA FQRRAGGVLA ISYLQGFLET ARLALHHLA |
| Biological Activity: | The ED50 as determined by a cell proliferation assay using murine NFS-60 cells is < 0.05 ng/ml, corresponding to a Specific Activity of 2 x 107IU/mg. |
Granulocyte Colony Stimulating Factor is a growth factor and/or cytokine produced by the endothelium, macrophages and a number of other immune cells. GCSF stimulates the bone marrow to produce granulocytes and also to stimulate the survival, proliferation, differentiation and function of neutrophil granulocyte progenator cells and mature neutrophils.
Granulocyte Colony Stimulating Factor Mouse Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 178 amino acids and having a molecular mass of approximately 18.9kDa. G-CSF is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | G-CSF: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell cycle regulation; Secreted; Cytokine; Secreted, signal peptide Cellular Component: extracellular space; extracellular region Molecular Function:enzyme binding; growth factor activity; cytokine activity Biological Process: positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of myeloid cell differentiation; positive regulation of actin filament polymerization; positive regulation of protein binding; granulocyte differentiation; positive regulation of cell proliferation; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; immune response; positive regulation of peptidyl-serine phosphorylation |
| UniProt Code: | P09920 |
| NCBI GenInfo Identifier: | 117565 |
| NCBI Gene ID: | 12985 |
| NCBI Accession: | P09920.1 |
| UniProt Related Accession: | P09920 |
| Molecular Weight: | 22,421 Da |
| NCBI Full Name: | Granulocyte colony-stimulating factor |
| NCBI Synonym Full Names: | colony stimulating factor 3 (granulocyte) |
| NCBI Official Symbol: | Csf3 |
| NCBI Official Synonym Symbols: | Csfg; G-CSF; MGI-IG |
| NCBI Protein Information: | granulocyte colony-stimulating factor |
| UniProt Protein Name: | Granulocyte colony-stimulating factor |
| Protein Family: | Granulocyte colony-stimulating factor |
| UniProt Gene Name: | Csf3 |
| UniProt Entry Name: | CSF3_MOUSE |
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