The Mouse G-CSF (Granulocyte Colony Stimulating Factor) ELISA Kit is specifically designed for the accurate measurement of G-CSF levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.G-CSF is a key cytokine that regulates the production and function of granulocytes, crucial components of the immune system. It plays a vital role in promoting the growth and differentiation of granulocyte progenitor cells, making it a valuable biomarker for studying immune responses and inflammatory processes.
This ELISA kit provides researchers with a powerful tool for studying G-CSF levels in mouse models, allowing for a better understanding of immune regulation, hematopoiesis, and inflammatory disorders. With its high-quality components and straightforward protocol, this kit is an essential resource for any laboratory conducting research in immunology, hematology, or inflammation.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse G-CSF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse G-CSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse G-CSF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse G-CSF. You can calculate the concentration of Mouse G-CSF in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
85-98
86-100
Average (%)
91
91
93
1:4
Range (%)
96-109
83-93
94-107
Average (%)
101
88
101
1:8
Range (%)
94-110
82-95
91-105
Average (%)
101
89
97
1:16
Range (%)
98-111
82-95
96-113
Average (%)
104
87
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-107
97
EDTA plasma (n=5)
86-99
92
Cell culture media (n=5)
89-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
52.2
95.2
375.5
48.4
104.4
403.7
Standard deviation
3.3
5.6
19.5
2.9
5.1
20.2
C V (%)
6.32
5.88
5.19
5.99
4.89
5.0
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse G-CSF concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse G-CSF in samples. No significant cross-reactivity or interference between Mouse G-CSF and analogues was observed.
