The Mouse FX (Coagulation Factor X) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of Coagulation Factor X levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it suitable for a wide range of research applications.Coagulation Factor X is a vital protein in the blood coagulation cascade, playing a crucial role in the initiation and propagation of thrombin generation.
Dysregulation of Coagulation Factor X can lead to bleeding disorders or thrombotic events, making it a key biomarker for studying hemostasis and thrombosis-related conditions.This Mouse FX CLIA Kit is essential for researchers studying coagulation disorders, thrombosis, and other related conditions in mouse models, providing valuable insights into the mechanisms underlying these diseases and potential therapeutic interventions.
Product Name:
Mouse FX (coagulation factor X) CLIA Kit
SKU:
MOES00185
Target:
Mouse FX (coagulation factor X)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
37.50 ng/mL
Detection range:
62.50-4000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse F10. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse F10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse F10, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse F10. You can calculate the concentration of Mouse F10 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-100
100-116
93-104
Average (%)
95
106
98
1:4
Range (%)
101-120
91-104
98-111
Average (%)
109
97
105
1:8
Range (%)
99-114
91-101
85-96
Average (%)
107
96
91
1:16
Range (%)
96-112
95-110
86-97
Average (%)
103
102
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-98
91
EDTA plasma (n=5)
95-109
102
Cell culture media (n=5)
97-110
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
183.85
525.73
1892.66
170.18
509.31
2018.86
Standard deviation
19.27
51.31
201.19
17.41
54.85
210.37
C V (%)
10.48
9.76
10.63
10.23
10.77
10.42
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse F10 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse F10 in samples. No significant cross-reactivity or interference between Mouse F10 and analogues was observed.
