The Mouse FVIII (Coagulation Factor VIII) ELISA Kit is specifically designed for the accurate measurement of coagulation Factor VIII levels in mouse serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring dependable and reproducible results for various research applications.Coagulation Factor VIII is a key protein involved in the blood clotting cascade, playing a crucial role in hemostasis.
Dysregulation of Factor VIII levels can lead to bleeding disorders such as hemophilia A, making it an important biomarker for studying these conditions and developing therapeutic interventions.With easy-to-follow protocols and rapid assay times, the Mouse FVIII ELISA Kit from Assay Genie provides researchers with a valuable tool for studying coagulation disorders and potential treatment strategies in mouse models.
Product Name:
Mouse FVIII (Coagulation Factor VIII) ELISA Kit
SKU:
MOES00873
Target:
Mouse FVIII (Coagulation Factor VIII)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse F8. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse F8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse F8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse F8. You can calculate the concentration of Mouse F8 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-100
86-100
86-101
Average (%)
95
93
92
1:4
Range (%)
88-101
84-97
85-98
Average (%)
95
90
92
1:8
Range (%)
89-104
79-93
89-102
Average (%)
95
85
94
1:16
Range (%)
89-103
81-93
88-101
Average (%)
94
87
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-109
102
EDTA plasma (n=5)
90-101
96
Cell culture media (n=5)
88-101
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.03
3.17
9.68
1.01
2.93
10.02
Standard deviation
0.05
0.15
0.48
0.06
0.17
0.53
C V (%)
4.85
4.73
4.96
5.94
5.8
5.29
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse F8 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse F8 in samples. No significant cross-reactivity or interference between Mouse F8 and analogues was observed.
