The Mouse Fusion (FUS) ELISA Kit is specifically designed for the precise quantification of FUS levels in mouse serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit ensures dependable and consistent results, catering to a variety of research needs.FUS is a key protein known for its role in gene regulation and RNA processing, making it essential for various cellular functions. Dysregulation of FUS has been linked to neurodegenerative diseases such as ALS, highlighting its importance as a biomarker for studying these conditions and exploring new therapeutic options.
Get accurate and reliable data with the Mouse Fusion (FUS) ELISA Kit, empowering your research efforts in unraveling the complexities of FUS biology and its implications in disease pathology. Visit our website to learn more and elevate your research to new heights.
Product Name:
Mouse Fusion (FUS) ELISA Kit
Product Code:
MODL00508
Size:
96 Assays
Target:
FUS
Synonyms:
CHOP, ALS6, TLS, hnRNP-P2, Amyotrophic Lateral Sclerosis 6, Heterogeneous Nuclear Ribonucleoprotein P2, 75 kDa DNA-pairing protein, Translocated in liposarcoma protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
