Mouse FST Recombinant Protein (RPPB0265)
- SKU:
- RPPB0265
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Mouse
- Target:
- FST
- Synonyms:
- Follistatin
- FST
- FS
- Activin-binding protein
- Source:
- Escherichia Coli
- Uniprot:
- P47931
Description
| Product Name: | Mouse FST Recombinant Protein |
| Product Code: | RPPB0265 |
| Size: | 10µg |
| Species: | Mouse |
| Target: | FST |
| Synonyms: | Follistatin, FST, FS, Activin-binding protein, AL033346. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The Mouse Follistatin is lyophilized from 10mM Na2PO4 and 50mM NaCl, pH 7.5. |
| Solubility: | It is recommended to reconstitute the lyophilized Follistatin in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Follistatin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FST should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGNCWLRQAK NGRCQVLTKT ELSKEECCST GRLSTSWTEE DVNDNTLFKW MIFNGGAPNC IPCKETCENV DCGPGKKCRM NKKNKPRCVC APDCSNITWK GPVCGLDGKT YRNECALLKA RCKEQPELEV QYQGRCKKTC RDVFCPGSST CVVDQTNNAY CVTCNRICPE PASSEQYLCG NDGVTYSSAC HLRKATCLLG RSIGLAYEGK CIKAKSCEDI QCTGGKKCLW DS |
| Biological Activity: | The ED50, determined by the dose-dependent neutralization of 7.5ng/ml human Activin-A on MCP-11 cells, is 0.13-0.19µg/ml. |
Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin. Follistatin binds directly to activin and functions as an activin antagonist. specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (fsh).
Follistatin Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 289 amino acids and having a total molecular mass of 31.6kDa.The FST is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | FST: Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH) |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted Cellular Component: cytoplasm; extracellular region; nucleus Molecular Function:heparan sulfate proteoglycan binding; activin binding Biological Process: BMP signaling pathway; positive regulation of hair follicle development; negative regulation of activin receptor signaling pathway; negative regulation of cell differentiation; hair follicle morphogenesis; gamete generation; hemopoietic progenitor cell differentiation; keratinocyte proliferation; negative regulation of transcription from RNA polymerase II promoter; pattern specification process; skeletal development; female gonad development; odontogenesis of dentine-containing teeth |
| NCBI Summary: | The protein encoded by this gene binds to and negatively regulates activin, as well as other members of the transforming growth factor beta family, and acts to prevent uncontrolled cellular proliferation. This protein also contains a heparin-binding sequence. It is expressed in many of the tissues in which activin is synthesized and is thought to clear activin from the circulation by attachment to the cell surface. Alternative splicing results in multiple transcript variants that encode multiple protein isoforms, including FST315 and FST288, that differ at their C-terminus. Another isoform, FST303 is thought to be produced by proteolytic cleavage of FST315. These isoforms differ in their localization and in their ability to bind heparin. While FST315 is a circulating protein, FST288 is tissue-bound, and FST303 is gonad-specific. While deletion of all isoforms results in embryonic lethality, expression of just FST288 is sufficient for embryonic development, but the resultant mice have fertility defects. [provided by RefSeq, Aug 2014] |
| UniProt Code: | P47931 |
| NCBI GenInfo Identifier: | 672890584 |
| NCBI Gene ID: | 14313 |
| NCBI Accession: | NP_001288302.1 |
| UniProt Related Accession: | P47931 |
| Molecular Weight: | 31.6 |
| NCBI Full Name: | follistatin FST315 |
| NCBI Synonym Full Names: | follistatin |
| NCBI Official Symbol: | Fst |
| NCBI Official Synonym Symbols: | FS; AL033346 |
| NCBI Protein Information: | follistatin |
| UniProt Protein Name: | Follistatin |
| UniProt Synonym Protein Names: | Activin-binding protein |
| Protein Family: | Follistatin |
| UniProt Gene Name: | Fst |
| UniProt Entry Name: | FST_MOUSE |
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