The Mouse FST (Follistatin) ELISA Kit is specifically designed for the precise measurement of follistatin levels in mouse serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers consistently accurate and reproducible results, making it a valuable tool for various research purposes.Follistatin is a key modulator of various biological processes, including muscle growth, inflammation, and reproduction.
By regulating the activity of certain growth factors, follistatin plays a crucial role in maintaining homeostasis and influencing diverse physiological functions. This makes it a significant biomarker in the study of conditions such as muscle disorders, inflammatory diseases, and infertility, offering insights for potential therapeutic interventions.
Product Name:
Mouse FST (Follistatin) ELISA Kit
SKU:
MOES01034
Target:
Mouse FST (Follistatin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse FST. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FST and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FST, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse FST. You can calculate the concentration of Mouse FST in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
86-98
90-100
Average (%)
95
92
95
1:4
Range (%)
90-103
86-100
83-93
Average (%)
95
92
87
1:8
Range (%)
88-101
83-96
85-99
Average (%)
93
89
91
1:16
Range (%)
94-105
81-91
88-99
Average (%)
99
86
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
99
EDTA plasma (n=5)
92-104
98
Cell culture media (n=5)
87-100
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.97
2.41
9.65
0.91
2.56
9.3
Standard deviation
0.07
0.14
0.51
0.05
0.11
0.34
C V (%)
7.22
5.81
5.28
5.49
4.3
3.66
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse FST concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse FST in samples. No significant cross-reactivity or interference between Mouse FST and analogues was observed.
