The Mouse FIX (Factor IX) Coagulation CLIA Kit is specifically designed for the quantitative measurement of mouse Factor IX levels in serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing accurate and reproducible results for various research applications.Factor IX is an important protein involved in the blood coagulation cascade, playing a crucial role in the clotting process.
Abnormalities in Factor IX levels can lead to bleeding disorders such as hemophilia, making it a key biomarker for studying these conditions and developing therapeutic interventions.With its user-friendly protocol and reliable performance, the Mouse FIX Coagulation CLIA Kit is a valuable tool for researchers seeking to investigate coagulation disorders and develop novel treatments.
Product Name:
Mouse FIX (Coagulation Factor IX) CLIA Kit
SKU:
MOES00184
Target:
Mouse FIX (Coagulation Factor IX)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse F9. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse F9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse F9, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse F9. You can calculate the concentration of Mouse F9 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
100-115
91-107
87-101
Average (%)
107
98
94
1:4
Range (%)
98-116
85-96
101-116
Average (%)
106
91
109
1:8
Range (%)
102-117
90-106
89-104
Average (%)
110
97
96
1:16
Range (%)
87-102
98-115
100-115
Average (%)
93
105
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
95
EDTA plasma (n=5)
89-101
94
Cell culture media (n=5)
99-113
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
91.7
258.28
824.6
99.06
233.95
752.79
Standard deviation
9.9
21.28
72.81
12.61
25.13
81.53
C V (%)
10.8
8.24
8.83
12.73
10.74
10.83
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse F9 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse F9 in samples. No significant cross-reactivity or interference between Mouse F9 and analogues was observed.
