Mouse FGF 21 Recombinant Protein (RPPB0245)
- SKU:
- RPPB0245
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Mouse
- Target:
- FGF 21
- Synonyms:
- Fibroblastgrowth factor 21
- FGF-21
- Source:
- Sf9 Insect cells
- Uniprot:
- Q9JJN1
Description
| Product Name: | Mouse FGF 21 Recombinant Protein |
| Product Code: | RPPB0245 |
| Size: | 10µg |
| Species: | Mouse |
| Target: | FGF 21 |
| Synonyms: | Fibroblastgrowth factor 21, FGF-21. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile Filtered colorless clear solution. |
| Formulation: | FGF-21 protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | AYPIPDSSPL LQFGGQVRQR YLYTDDDQDT EAHLEIREDG TVVGAAHRSPESLLELKALK PGVIQILGVK ASRFLCQQPD GALYGSPHFD PEACSFRELL LEDGYNVYQS EAHGLPLRLPQKDSPNQDAT SWGPVRFLPM PGLLHEPQDQ AGFLPPEPPD VGSSDPLSMV EPLQGRSPSYASLEHHHHHH |
The FGFs are a family of more than 20 small (~17–26 kDa) secreted peptides. The initial characterization of these proteins focused on their ability to stimulate fibroblast proliferation. This mitogenic activity was mediated through FGF receptors (FGFRs) 1, 2, or 3. A fourth closely related tyrosine kinase receptor (FGFR4) was able to bind the FGFs but did not lead to a mitogenic response.FGFs modulate cellular activity via at least 5 distinct subfamilies of high-affinity FGF receptors (FGFRs): FGFR-1, -2, -3, and -4, all with intrinsic tyrosine kinase activity and, except for FGFR-4, multiple splice isoforms, and FGFR-5, which lacks an intracellular kinase domain. There is growing evidence that FGFRs can be important for regulation of glucose and lipid homeostasis. The overexpression of a dominant negative form of FGFR-1 in cells leads to diabetes in mice, which thus implies that proper FGF signaling is required for normal cell function and glycemia maintenance. FGFR-2 appears to be a key molecule during pancreatic development. Moreover, FGFR-4 has been implicated in cholesterol metabolism and bile acid synthesis.FGF-21 is preferentially expressed in liver, but an exact knowledge of FGF-21 bioactivity and its mode of action have been lacking to date. FGF-21 is a potent activator of glucose uptake on adipocytes, protects animals from diet-induced obesity when overexpressed in transgenic mice, and lowers blood glucose and triglyceride levels when therapeutically administered to diabetic rodents.
FGF-21 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 190 amino acids (29-210a.a.) and having a molecular mass of 21.0kDa (Molecular size on SDS-PAGE will appear at approximately 18-28kDa). FGF21 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | FGF21: Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Belongs to the heparin-binding growth factors family. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted Cellular Component: extracellular region; extracellular space Molecular Function:fibroblast growth factor receptor binding; growth factor activity; receptor binding Biological Process: fibroblast growth factor receptor signaling pathway; positive regulation of cell proliferation; positive regulation of glucose import |
| UniProt Code: | Q9JJN1 |
| NCBI GenInfo Identifier: | 13626695 |
| NCBI Gene ID: | 105242481 |
| NCBI Accession: | Q9JJN1.1 |
| Molecular Weight: | 23,237 Da |
| NCBI Full Name: | Fibroblast growth factor 21 |
| NCBI Official Symbol: | LOC105242481 |
| NCBI Protein Information: | fibroblast growth factor 21 |
| UniProt Protein Name: | Fibroblast growth factor 21 |
| Protein Family: | Fibroblast growth factor |
| UniProt Gene Name: | Fgf21 |
| UniProt Entry Name: | FGF21_MOUSE |
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