The Mouse FGA (Fibrinogen Alpha Chain) CLIA Kit is specifically designed for the quantification of Fibrinogen Alpha Chain levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for various research applications. Fibrinogen Alpha Chain is an essential component of the blood clotting process, playing a critical role in hemostasis and wound healing. Abnormal levels of Fibrinogen Alpha Chain have been associated with various inflammatory diseases, cardiovascular disorders, and liver conditions, highlighting its importance as a biomarker for disease progression and treatment efficacy.
With its reliable performance and easy-to-use format, the Mouse FGA CLIA Kit is a valuable tool for researchers studying coagulation disorders, thrombotic events, and inflammatory responses in mouse models. Trust in this kit to deliver accurate and insightful data for your experimental needs.
Product Name:
Mouse FGA (Fibrinogen Alpha chain) CLIA Kit
SKU:
MOES00259
Target:
Mouse FGA (Fibrinogen Alpha chain)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
3.75 ng/mL
Detection range:
6.25-400 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse FGα. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FGα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FGα, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse FGα. You can calculate the concentration of Mouse FGα in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-109
95-111
98-113
Average (%)
102
102
104
1:4
Range (%)
98-113
90-106
92-107
Average (%)
104
98
98
1:8
Range (%)
93-106
94-108
90-102
Average (%)
99
101
95
1:16
Range (%)
86-101
102-114
97-110
Average (%)
93
108
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
97
EDTA plasma (n=5)
88-100
94
Cell culture media (n=5)
94-107
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
20.66
63.23
175.23
21.27
57.22
179.82
Standard deviation
1.82
5.47
18.71
2.0
6.19
12.35
C V (%)
8.81
8.65
10.68
9.4
10.82
6.87
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse FGα concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse FGα in samples. No significant cross-reactivity or interference between Mouse FGα and analogues was observed.
