FTH1,FTHL6,PIG15,PLIF,Ferritin Heavy Chain,Apoferritin,Placenta Immunoregulatory Factor,Proliferation-Inducing Protein 15,Cell proliferation-inducing gene 15 protein
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
The Mouse Ferritin Heavy Polypeptide (FTH) ELISA Kit is a reliable and accurate tool for measuring levels of FTH in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides trustworthy and consistent results, making it suitable for various research applications.FTH is a key protein involved in iron storage and regulation, playing a crucial role in maintaining iron homeostasis within the body. Dysregulation of FTH levels has been linked to conditions such as iron overload disorders, inflammation, and neurodegenerative diseases, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.
Overall, the Mouse FTH ELISA Kit offers researchers a valuable tool for studying the role of FTH in various physiological and pathological processes, providing insights into potential mechanisms and targets for future research and drug development efforts.
Product Name:
Mouse Ferritin, Heavy Polypeptide (FTH) ELISA Kit
Product Code:
MODL00506
Size:
96 Assays
Target:
FTH
Synonyms:
FTH1, FTHL6, PIG15, PLIF, Ferritin Heavy Chain, Apoferritin, Placenta Immunoregulatory Factor, Proliferation-Inducing Protein 15, Cell proliferation-inducing gene 15 protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
