The Mouse Fe-Ferritin ELISA Kit is specifically designed for the precise quantification of Fe-ferritin levels in mouse serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.Fe-ferritin is a vital protein responsible for iron storage and regulation in the body. Abnormal levels of Fe-ferritin have been linked to various health conditions, including iron deficiency anemia, hemochromatosis, and neurodegenerative diseases.
As such, measuring Fe-ferritin levels can provide valuable insights into these conditions and aid in the development of potential treatments. The Mouse Fe-Ferritin ELISA Kit is a valuable tool for researchers studying iron metabolism, red blood cell production, and iron-related disorders in mice. Its user-friendly protocol and high performance make it an essential asset for laboratories conducting preclinical studies and drug development research.
Product Name:
Mouse FE (Ferritin) ELISA Kit
SKU:
MOES01014
Target:
Mouse FE (Ferritin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse FE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse FE. You can calculate the concentration of Mouse FE in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
90-103
85-97
Average (%)
95
98
91
1:4
Range (%)
98-112
85-100
93-106
Average (%)
105
92
101
1:8
Range (%)
95-111
87-103
92-105
Average (%)
103
94
99
1:16
Range (%)
98-111
85-97
95-112
Average (%)
105
90
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
97
EDTA plasma (n=5)
89-105
96
Cell culture media (n=5)
90-104
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.8
14.1
41.1
4.5
15.4
41.3
Standard deviation
0.3
0.6
1.6
0.3
0.8
1.4
C V (%)
6.25
4.26
3.89
6.67
5.19
3.39
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse FE concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse FE in samples. No significant cross-reactivity or interference between Mouse FE and analogues was observed.
