The Mouse FBN1 (Fibrillin-1) CLIA Kit is a reliable tool for accurately measuring Fibrillin-1 levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures consistent and precise results for a variety of research purposes. Fibrillin-1 is a key component of microfibrils in the extracellular matrix, playing a crucial role in maintaining tissue structure and elasticity. Mutations in the FBN1 gene have been associated with various genetic disorders, including Marfan syndrome, making Fibrillin-1 a valuable biomarker for researching these conditions and potential therapeutic interventions.
Whether investigating connective tissue disorders, cardiovascular diseases, or developmental abnormalities, the Mouse FBN1 CLIA Kit provides researchers with a powerful tool for studying the role of Fibrillin-1 in health and disease.
Product Name:
Mouse FBN1 (Fibrillin 1) CLIA Kit
SKU:
MOES00258
Target:
Mouse FBN1 (Fibrillin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
1.88 pg/mL
Detection range:
3.13-200 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse FBN1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FBN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FBN1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse FBN1. You can calculate the concentration of Mouse FBN1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-107
95-109
86-102
Average (%)
100
102
93
1:4
Range (%)
96-111
96-111
90-104
Average (%)
102
104
97
1:8
Range (%)
97-114
86-97
94-108
Average (%)
104
92
102
1:16
Range (%)
91-105
100-116
87-101
Average (%)
98
108
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-114
107
EDTA plasma (n=5)
96-109
102
Cell culture media (n=5)
92-109
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.54
31.37
95.65
10.66
32.59
91.88
Standard deviation
1.34
2.42
6.29
1.26
3.75
8.42
C V (%)
12.71
7.71
6.58
11.82
11.51
9.16
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse FBN1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse FBN1 in samples. No significant cross-reactivity or interference between Mouse FBN1 and analogues was observed.
