The Mouse Fibrinogen ELISA Kit is specifically designed for the precise detection of fibrinogen levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing dependable and consistent results, making it perfect for various research applications.Fibrinogen is a key protein involved in blood clotting, crucial for wound healing and maintaining vascular integrity. Abnormal levels of fibrinogen are associated with various health conditions such as cardiovascular diseases, inflammatory disorders, and liver diseases, making it a valuable biomarker for studying these conditions and exploring potential treatments.
This Mouse Fibrinogen ELISA Kit provides a convenient and reliable tool for researchers to accurately measure fibrinogen levels in mouse samples, facilitating in-depth investigations into the role of fibrinogen in disease pathogenesis and progression. Ensure the success of your research with this high-quality ELISA kit.
Product Name:
Mouse Fbg (Fibrinogen) ELISA Kit
SKU:
MOES01021
Target:
Mouse Fbg (Fibrinogen)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
9.38 ng/mL
Detection range:
15.63-1000 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse FG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse FG. You can calculate the concentration of Mouse FG in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-99
92-103
86-101
Average (%)
93
98
92
1:4
Range (%)
90-100
83-94
82-96
Average (%)
95
89
88
1:8
Range (%)
89-105
82-95
82-94
Average (%)
96
88
87
1:16
Range (%)
92-105
83-93
84-98
Average (%)
98
88
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-111
101
EDTA plasma (n=5)
95-111
101
Cell culture media (n=5)
88-99
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
47.25
147.13
350.41
50.54
149.11
372.71
Standard deviation
2.52
7.84
15.87
2.74
6.25
15.73
C V (%)
5.33
5.33
4.53
5.42
4.19
4.22
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse FG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse FG in samples. No significant cross-reactivity or interference between Mouse FG and analogues was observed.
