Mouse Fatty Acid Binding Protein 4, Adipocyte (FABP4) ELISA Kit

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SKU:
MODL00446
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P04117
Sensitivity:
14.5pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
A-FABP,AFABP,AP2,Adipocyte Protein 2,Adipocyte-type fatty acid-binding protein
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates And Other Biological Fluids
Frequently bought together:

Description

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Mouse Fatty Acid Binding Protein 4, Adipocyte (FABP4) ELISA Kit

The Mouse Fatty Acid Binding Protein 4 (Adipocyte FABP4) ELISA Kit is a reliable and precise tool for measuring levels of FABP4 in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and reproducible results, making it suitable for various research applications.FABP4, also known as adipocyte Fatty Acid Binding Protein, is a key protein involved in lipid metabolism and insulin resistance.

Elevated FABP4 levels have been associated with obesity, diabetes, and cardiovascular diseases, highlighting its importance as a biomarker for these conditions. By measuring FABP4 levels, researchers can gain valuable insights into the mechanisms underlying metabolic disorders and develop potential therapeutic interventions. Order the Mouse FABP4 ELISA Kit today and unlock the potential for groundbreaking discoveries in metabolic research.

Product Name:Mouse Fatty Acid Binding Protein 4, Adipocyte (FABP4) ELISA Kit
Product Code:MODL00446
Size:96 Assays
Target:FABP4
Synonyms:A-FABP, AFABP, AP2, Adipocyte Protein 2, Adipocyte-type fatty acid-binding protein
Detection Method:Sandwich
Range:31.25-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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