Mouse Fatty Acid Binding Protein 3, Muscle And Heart (FABP3) ELISA Kit

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SKU:
MODL00445
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P11404
Sensitivity:
26.3pg/mL
Range:
62.5-4000pg/mL
ELISA Type:
Sandwich
Synonyms:
MDGI,FABP11,H-FABP,O-FABP,Hfabp,Mammary Derived Growth Inhibitor,Fatty Acid Binding Protein 11,Muscle fatty acid-binding protein,Heart-type fatty acid-binding protein
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
Frequently bought together:

Description

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Mouse Fatty Acid Binding Protein 3, Muscle And Heart (FABP3) ELISA Kit

The Mouse Fatty Acid Binding Protein 3 (Muscle and Heart) ELISA Kit is specifically designed for the accurate measurement of FABP3 levels in mouse serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, providing reliable and reproducible results for a variety of research applications.FABP3, also known as heart fatty acid-binding protein, is a key protein involved in fatty acid metabolism in muscle and heart tissues. It plays a critical role in lipid transport and cellular energy metabolism, making it a valuable biomarker for studying metabolic disorders, cardiovascular diseases, and skeletal muscle function.

By utilizing the Mouse FABP3 ELISA Kit, researchers can gain valuable insights into the role of FABP3 in various physiological and pathological processes, ultimately leading to a better understanding of metabolic diseases and potential therapeutic interventions.

Product Name:Mouse Fatty Acid Binding Protein 3, Muscle And Heart (FABP3) ELISA Kit
Product Code:MODL00445
Size:96 Assays
Target:FABP3
Synonyms:MDGI, FABP11, H-FABP, O-FABP, Hfabp, Mammary Derived Growth Inhibitor, Fatty Acid Binding Protein 11, Muscle fatty acid-binding protein, Heart-type fatty acid-binding protein
Detection Method:Sandwich
Range:62.5-4000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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