Mouse FASL (Factor Related Apoptosis Ligand) CLIA Kit
The Mouse Fasl (Factor Related Apoptosis Ligand) CLIA Kit is a powerful tool for the quantitative measurement of Fasl levels in mouse samples. This kit is specifically designed for accurate detection in serum, plasma, and cell culture supernatants with high sensitivity and specificity, ensuring dependable and consistent results for a variety of research applications.Fasl, also known as CD95L, is a critical protein involved in the regulation of cell death and immune responses. Its dysregulation has been implicated in various diseases, including autoimmune disorders and cancer.
The Mouse Fasl CLIA Kit provides researchers with a valuable tool for studying the role of Fasl in these conditions and developing potential therapeutic interventions.Overall, the Mouse Fasl CLIA Kit is a reliable and efficient solution for researchers looking to assess Fasl levels in mouse samples accurately and efficiently. Its high-performance features make it an essential tool for advancing our understanding of cell death mechanisms and developing new treatments for related diseases.
Product Name:
Mouse FASL (Factor Related Apoptosis Ligand) CLIA Kit
SKU:
MOES00025
Target:
Mouse FASL (Factor Related Apoptosis Ligand)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse FASL. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FASL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FASL, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse FASL. You can calculate the concentration of Mouse FASL in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
102-120
91-104
Average (%)
93
109
96
1:4
Range (%)
86-100
97-114
102-119
Average (%)
93
105
110
1:8
Range (%)
92-108
93-104
88-101
Average (%)
99
99
95
1:16
Range (%)
95-110
97-107
92-104
Average (%)
103
102
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
95
EDTA plasma (n=5)
95-106
100
Cell culture media (n=5)
95-109
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
40.13
91.07
273.64
37.76
96.3
275.85
Standard deviation
5.05
7.24
19.29
4.87
8.26
25.27
C V (%)
12.58
7.95
7.05
12.9
8.58
9.16
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse FASL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse FASL in samples. No significant cross-reactivity or interference between Mouse FASL and analogues was observed.
