Mouse FAS/CD95 (Factor Related Apoptosis) CLIA Kit
The Mouse Fas (CD95) Factor Related Apoptosis CLIA Kit is a reliable and accurate tool designed for the quantitative detection of Fas (CD95) levels in mouse serum, plasma, and tissue lysates. This kit offers high sensitivity and specificity, allowing for precise and reproducible results in a variety of research settings.Fas (CD95) is a key protein involved in apoptosis, or programmed cell death, playing a critical role in immune regulation and tissue homeostasis. Dysregulation of Fas signaling has been implicated in various diseases, including autoimmune disorders, cancer, and neurodegenerative conditions, making it a valuable target for therapeutic interventions and biomarker research.
With its advanced technology and validated performance, the Mouse Fas (CD95) Factor Related Apoptosis CLIA Kit is an essential tool for researchers investigating the molecular mechanisms of apoptosis and developing novel therapeutic strategies for Fas-related diseases.
Product Name:
Mouse FAS/CD95 (Factor Related Apoptosis) CLIA Kit
SKU:
MOES00253
Target:
Mouse FAS/CD95 (Factor Related Apoptosis)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse FAS/CD95. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FAS/CD95 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FAS/CD95, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse FAS/CD95. You can calculate the concentration of Mouse FAS/CD95 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
86-100
102-116
Average (%)
99
92
108
1:4
Range (%)
91-101
91-105
95-106
Average (%)
96
97
100
1:8
Range (%)
85-96
86-98
97-111
Average (%)
91
91
105
1:16
Range (%)
87-98
101-117
84-95
Average (%)
93
108
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-101
93
EDTA plasma (n=5)
84-99
91
Cell culture media (n=5)
87-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
40.4
74.61
274.61
42.39
73.56
281.57
Standard deviation
5.22
8.65
30.67
4.57
5.49
20.67
C V (%)
12.92
11.59
11.17
10.78
7.46
7.34
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse FAS/CD95 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse FAS/CD95 in samples. No significant cross-reactivity or interference between Mouse FAS/CD95 and analogues was observed.
