Mouse FABP6 (Fatty Acid Binding Protein 6, Ileal) CLIA Kit
The Mouse FABP6 (Fatty Acid Binding Protein 6) Ileal CLIA Kit is a powerful tool for the accurate measurement of FABP6 levels in mouse samples. FABP6 is a critical protein involved in the transportation of fatty acids in the small intestine, playing a crucial role in lipid metabolism and absorption.This CLIA kit offers high sensitivity and specificity, ensuring precise and reliable results for your research needs. Whether studying the role of FABP6 in gut health, metabolic disorders, or other conditions, this kit provides a valuable tool for understanding the mechanisms of fatty acid transport and absorption in the body.
With easy-to-follow protocols and a user-friendly format, the Mouse FABP6 Ileal CLIA Kit from Assay Genie is a trusted choice for researchers seeking accurate and efficient detection of FABP6 levels in mouse samples.
Product Name:
Mouse FABP6 (Fatty Acid Binding Protein 6, Ileal) CLIA Kit
SKU:
MOES00255
Target:
Mouse FABP6 (Fatty Acid Binding Protein 6, Ileal)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse FABP6. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FABP6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FABP6, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse FABP6. You can calculate the concentration of Mouse FABP6 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-112
87-98
89-101
Average (%)
102
92
95
1:4
Range (%)
84-96
98-112
102-118
Average (%)
91
106
109
1:8
Range (%)
93-106
88-102
93-106
Average (%)
99
94
100
1:16
Range (%)
98-112
94-107
87-99
Average (%)
106
101
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-105
99
EDTA plasma (n=5)
92-108
98
Cell culture media (n=5)
91-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
53.65
137.8
448.89
58.3
145.94
474.59
Standard deviation
5.1
14.28
32.32
5.4
16.0
39.11
C V (%)
9.51
10.36
7.2
9.26
10.96
8.24
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse FABP6 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse FABP6 in samples. No significant cross-reactivity or interference between Mouse FABP6 and analogues was observed.
