Mouse FABP4 (Fatty Acid Binding Protein 4, Adipocyte) ELISA Kit
The Mouse FABP4 (Fatty Acid Binding Protein 4) Adipocyte ELISA Kit is specifically designed for the quantitative detection of FABP4 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for various research applications.FABP4 is a key protein involved in lipid metabolism and adipocyte function, playing a critical role in regulating glucose and lipid homeostasis.
Dysregulation of FABP4 has been linked to obesity, diabetes, and cardiovascular diseases, making it a valuable biomarker for studying metabolic disorders and developing potential therapeutic interventions.With its user-friendly features and high-performance capabilities, the Mouse FABP4 Adipocyte ELISA Kit is an essential tool for researchers studying adipocyte biology, metabolic disorders, and potential therapeutic targets in mouse models.
Product Name:
Mouse FABP4 (Fatty Acid Binding Protein 4, Adipocyte) ELISA Kit
SKU:
MOES01685
Target:
Mouse FABP4 (Fatty Acid Binding Protein 4, Adipocyte)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse FABP4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FABP4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FABP4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse FABP4. You can calculate the concentration of Mouse FABP4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-107
98-112
96-108
Average (%)
98
106
101
1:4
Range (%)
88-102
82-93
89-101
Average (%)
94
87
94
1:8
Range (%)
89-101
86-99
84-97
Average (%)
94
92
89
1:16
Range (%)
91-106
81-94
90-102
Average (%)
97
87
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-101
95
EDTA plasma (n=5)
86-100
92
Cell culture media (n=5)
86-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.44
6.75
24.98
2.57
6.44
23.51
Standard deviation
0.17
0.36
1.11
0.17
0.29
0.71
C V (%)
6.97
5.33
4.44
6.61
4.5
3.02
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse FABP4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse FABP4 in samples. No significant cross-reactivity or interference between Mouse FABP4 and analogues was observed.
