Mouse FABP3 (Fatty Acid Binding Protein 3, Muscle and Heart) ELISA Kit
The Mouse FABP3 (Fatty Acid Binding Protein 3) ELISA Kit is specially designed for the precise measurement of FABP3 levels in mouse muscle and heart tissue samples. This high-quality kit offers excellent sensitivity and specificity, ensuring accurate and reproducible results for your research needs.FABP3 is a key protein involved in fatty acid metabolism and transport, playing a crucial role in energy production and utilization in muscle and heart tissues. Dysregulation of FABP3 has been linked to various metabolic disorders, making it a valuable biomarker for studying these conditions and developing targeted therapies.
Whether investigating cardiac function, muscle metabolism, or studying metabolic diseases, the Mouse FABP3 ELISA Kit provides a reliable tool for researchers seeking to understand the role of FABP3 in health and disease. Order yours today and unlock new insights into the molecular mechanisms underlying metabolic regulation in mouse models.
Product Name:
Mouse FABP3 (Fatty Acid Binding Protein 3, Muscle and Heart) ELISA Kit
SKU:
MOES01684
Target:
Mouse FABP3 (Fatty Acid Binding Protein 3, Muscle and Heart)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse FABP3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FABP3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FABP3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse FABP3. You can calculate the concentration of Mouse FABP3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-110
96-109
99-114
Average (%)
102
103
105
1:4
Range (%)
93-107
83-94
86-100
Average (%)
98
89
93
1:8
Range (%)
86-99
82-95
88-103
Average (%)
93
88
94
1:16
Range (%)
86-101
81-91
88-98
Average (%)
93
86
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
95
EDTA plasma (n=5)
90-104
95
Cell culture media (n=5)
95-110
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
214.88
430.91
1826.64
200.29
418.87
1774.01
Standard deviation
11.17
22.02
74.53
12.32
19.69
71.67
C V (%)
5.2
5.11
4.08
6.15
4.7
4.04
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse FABP3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse FABP3 in samples. No significant cross-reactivity or interference between Mouse FABP3 and analogues was observed.
