The Mouse F4/80 Monoclonal Antibody (FITC Conjugated, Cl: A3-1) (AGEL0566) from Assay Genie is a powerful tool for researchers studying immune cells in mouse models. This antibody specifically targets the F4/80 protein, a well-known marker for mouse macrophages, allowing for precise identification and analysis of these important immune cells.Macrophages play key roles in immune responses, inflammation, and tissue repair, making them a focus of research in immunology, cancer, and various disease contexts. By using the Mouse F4/80 Monoclonal Antibody, researchers can detect and track macrophages in tissues and blood samples, gaining insights into their functions and behaviors.
This antibody, conjugated with FITC for fluorescent detection, is highly sensitive and specific, ensuring reliable results in flow cytometry, immunofluorescence, and other experimental techniques. Its validation for use in mouse samples makes it an essential reagent for studies aiming to characterize and manipulate macrophages in diverse research settings.Overall, the Mouse F4/80 Monoclonal Antibody (FITC Conjugated, Cl: A3-1) from Assay Genie is a valuable tool for investigating macrophages and their roles in immunity, inflammation, and disease processes in mouse models.
Each lot of this antibody is quality control tested by flow cytometric analysis. Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use. We suggest each investigator should titrate the reagent to obtain optimal results [The recommended concentration is 0.1-1 μg/106 cells in 100 μL volume].
Spectrum:
Swissprot:
Q61549
Gene ID:
13733
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.