The Mouse ET-1 (Endothelin-1) CLIA Kit is specifically designed for the accurate measurement of endothelin-1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.Endothelin-1 is a key peptide involved in vascular regulation, exerting powerful vasoconstrictor effects and contributing to cardiovascular homeostasis. Dysregulation of endothelin-1 has been implicated in various diseases such as hypertension, heart failure, and pulmonary arterial hypertension, making it a crucial biomarker for studying these conditions and exploring potential therapeutic interventions.
With its advanced technology and easy-to-use format, the Mouse ET-1 CLIA Kit from Assay Genie is an essential tool for researchers investigating the role of endothelin-1 in health and disease. Get accurate and actionable results with this cutting-edge assay kit.
Product Name:
Mouse ET-1 (Endothelin 1) CLIA Kit
SKU:
MOES00243
Target:
Mouse ET-1 (Endothelin 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
1.88 pg/mL
Detection range:
3.13-200 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse ET-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ET-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ET-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse ET-1. You can calculate the concentration of Mouse ET-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
87-102
93-111
Average (%)
98
93
101
1:4
Range (%)
94-106
98-112
102-120
Average (%)
101
105
110
1:8
Range (%)
91-107
89-101
86-97
Average (%)
98
94
91
1:16
Range (%)
92-102
91-107
92-109
Average (%)
97
98
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-107
101
EDTA plasma (n=5)
93-108
101
Cell culture media (n=5)
100-115
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.7
23.36
90.21
10.8
23.62
94.16
Standard deviation
1.22
1.79
5.87
1.37
2.01
10.44
C V (%)
11.4
7.66
6.51
12.69
8.51
11.09
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse ET-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ET-1 in samples. No significant cross-reactivity or interference between Mouse ET-1 and analogues was observed.