EPHA1 or EPH receptor A1 belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. An important role of Eph receptors and their ligands ephrins is to mediate cell-contact-dependent repulsion. Eph receptors and ephrins also act at boundaries to channel neuronal growth cones along specific pathways, restrict the migration of neural crest cells, and via bidirectional signaling prevent intermingling between hindbrain segments. Eph receptors and ephrins can also trigger an adhesive response of endothelial cells and are required for the remodeling of blood vessels. Eph receptors and ephrins have emerged as key regulators of the repulsion and adhesion of cells that underlie the establishment, maintainence, and remodeling of patterns of cellular organization. The ephrins and Eph receptors are implicated as positional labels that may guide the development of neural topographic maps.
Product Name:
Mouse EphA1 Recombinant Protein (RPES1527)
Product Code:
RPES1527
Size:
100µg
Species:
Mouse
Expressed Host:
HEK293 Cells
Synonyms:
5730453L17Rik,AL033318,Eph,Esk
Accession:
Q60750
Sequence:
Met 1-Glu 548
Fusion tag:
C-His
Activity:
Measured by its binding ability in a functional ELISA. Immobilized mouse EPHA1-His at 10 µg/ml (100 ?l/well) can bind mouse EFNA1-Fc ), The EC50 of mouse EFNA1-Fc is 21.3-49.8 ng/ml.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the extracellular domain of mouse EPHA1 (Q60750) (Met 1-Glu 548) was expressed, with a C-terminal polyhistidine tag.
Purity:
> 97 % as determined by SDS-PAGE
Mol Mass:
58.6 kDa
AP Mol Mass:
68 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
