The Mouse ENG (Endoglin) ELISA Kit is specifically designed for the precise measurement of endoglin levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing consistent and accurate results, making it a valuable tool for various research purposes.Endoglin is a key protein involved in angiogenesis and vascular development, playing a critical role in regulating blood vessel growth and remodeling.
Dysregulation of endoglin has been linked to various diseases, including cancer, cardiovascular disorders, and inflammation, making it a significant biomarker for studying these conditions and exploring potential therapeutic interventions.With its user-friendly design and superior performance, the Mouse ENG (Endoglin) ELISA Kit is an essential tool for researchers investigating the role of endoglin in disease pathogenesis and exploring novel treatment strategies.
Product Name:
Mouse ENG (Endoglin) ELISA Kit
SKU:
MOES01486
Target:
Mouse ENG (Endoglin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse ENG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse ENG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse ENG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse ENG. You can calculate the concentration of Mouse ENG in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-108
85-97
85-97
Average (%)
99
91
90
1:4
Range (%)
91-109
82-96
86-101
Average (%)
99
88
93
1:8
Range (%)
89-103
82-94
87-100
Average (%)
96
88
94
1:16
Range (%)
93-107
87-100
81-95
Average (%)
98
92
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-106
98
EDTA plasma (n=5)
87-100
92
Cell culture media (n=5)
84-99
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
200.09
481.89
1354.99
218.52
515.11
1433.13
Standard deviation
10.62
22.07
70.19
13.18
24.83
54.89
C V (%)
5.31
4.58
5.18
6.03
4.82
3.83
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse ENG concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse ENG in samples. No significant cross-reactivity or interference between Mouse ENG and analogues was observed.
