The Mouse ENA-78 (Epithelial Neutrophil Activating peptide-78) CLIA Kit is a powerful tool for measuring ENA-78 levels in mouse serum, plasma, and cell culture supernatants. This kit provides exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.ENA-78 is a key chemokine involved in the recruitment and activation of neutrophils, playing a critical role in inflammatory responses. Elevated levels of ENA-78 have been associated with various inflammatory conditions, including arthritis, asthma, and inflammatory bowel disease, making it an important biomarker for studying these diseases and developing potential treatments.
With the Mouse ENA-78 CLIA Kit, researchers can confidently measure ENA-78 levels in mouse samples, providing valuable insights into the role of this chemokine in inflammatory processes and disease pathogenesis.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CXCL5. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CXCL5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CXCL5, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CXCL5. You can calculate the concentration of Mouse CXCL5 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-113
88-98
98-115
Average (%)
104
93
106
1:4
Range (%)
86-97
86-97
94-107
Average (%)
92
92
100
1:8
Range (%)
102-119
99-115
100-115
Average (%)
109
105
107
1:16
Range (%)
103-119
104-120
85-97
Average (%)
110
110
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-113
107
EDTA plasma (n=5)
99-115
106
Cell culture media (n=5)
86-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
22.96
53.9
216.83
24.66
57.59
217.74
Standard deviation
2.77
4.29
20.08
1.99
4.23
23.17
C V (%)
12.06
7.96
9.26
8.07
7.35
10.64
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CXCL5 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CXCL5 in samples. No significant cross-reactivity or interference between Mouse CXCL5 and analogues was observed.
