DPP7 (dipeptidylpeptidase 7), also known as DPPII and DPP2, is a post-proline cleaving aminopeptidase expressed in quiescent lymphocytes. Dipeptidyl peptidases (DPPs) have post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. DPPs mediate regulatory activity of their substrates and have been linked to a variety of diseases including type 2 diabetes, obesity and cancer. DPPs can bind specific voltage-gated potassium channels and alter their expression and biophysical properties and may also influence T cells. DPP proteins include DPRP1, DPRP2, DPP3, DPP7, DPP10, DPPX and CD26. It localizes to lysosomes. DPP7 localizes to lysosomes and exists as a homodimer via its leucine zipper motif and is involved in the degradation of oligopeptides. In response to calcium release, it can be secreted in its active form. It is essential for lymphocyte survival, as the inhibition of DPP7 results in quiescent cell apoptosis.
Product Name:
Mouse DPP7/DPPII/DPP2 Recombinant Protein (RPES1042)
Product Code:
RPES1042
Size:
10µg
Species:
Mouse
Expressed Host:
HEK293 Cells
Synonyms:
Dpp2,DPPII,QPP
Accession:
Q9ET221
Sequence:
Met1-Arg 506
Fusion tag:
C-His
Activity:
Measured by its ability to cleave the fluorogenic peptide substrate, Lys-Pro-AMC(KP-AMC).The specific activity is > 20,000 pmoles/min/µg.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mouse DPP7 (Q9ET221) (Met1-Arg 506) was expressed, with a C-terminal polyhistidine tag.
Purity:
> 92 % as determined by SDS-PAGE
Mol Mass:
53.7 kDa
AP Mol Mass:
65 kDa
Formulation:
Lyophilized from sterile PBS, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
