Mouse Divalent Metal Transporter 1 (DMT1) ELISA Kit
SKU:
MODL00382
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P49282
Sensitivity:
0.109ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
SLC11A2,DCT1,NRAMP2,Natural resistance-associated macrophage protein 2,Solute Carrier Family 11 Member 2,Proton-Coupled Divalent Metal Ion Transporters
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
Mouse Divalent Metal Transporter 1 (DMT1) ELISA Kit
The Mouse Divalent Metal Transporter 1 (DMT1) ELISA Kit is specifically designed for the accurate detection of DMT1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications. DMT1 is a key protein responsible for the transport of divalent metals such as iron and zinc across cell membranes.
It plays a crucial role in maintaining cellular homeostasis and is implicated in various diseases including iron-overload disorders and neurodegenerative conditions. The Mouse DMT1 ELISA Kit serves as a valuable tool for studying the function and regulation of DMT1 in physiological and pathological processes, providing insight into potential therapeutic strategies targeting divalent metal transport mechanisms.
Product Name:
Mouse Divalent Metal Transporter 1 (DMT1) ELISA Kit
Product Code:
MODL00382
Size:
96 Assays
Target:
DMT1
Synonyms:
SLC11A2, DCT1, NRAMP2, Natural resistance-associated macrophage protein 2, Solute Carrier Family 11 Member 2,Proton-Coupled Divalent Metal Ion Transporters
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
