Mouse DCN Recombinant Protein (RPPB3335)
- SKU:
- RPPB3335
- Product type:
- Recombinant Protein
- Size:
- 10ug
- Species:
- Mouse
- Target:
- DCN
- Synonyms:
- Decorin
- Bone proteoglycan II
- PG-S2
- PG40
- Source:
- Sf9 Insect cells
- Uniprot:
- P28654
Description
| Product Name: | Mouse DCN Recombinant Protein |
| Product Code: | RPPB3335 |
| Size: | 10µg |
| Species: | Mouse |
| Target: | DCN |
| Synonyms: | Decorin, Bone proteoglycan II, PG-S2, PG40, DCN. |
| Source: | Sf9 Insect cells |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | DCN protein solution (0.25mg/ml) contains Phosphate buffered saline (pH7.4), 30% glycerol and 0.1mM PMSF. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | GPFEQRGLFD FMLEDEASGI IPYDPDNPLI SMCPYRCQCH LRVVQCSDLG LDKVPWDFPP DTTLLDLQNN KITEIKEGAF KNLKDLHTLI LVNNKISKIS PEAFKPLVKL ERLYLSKNQL KELPEKMPRT LQELRVHENE ITKLRKSDFN GLNNVLVIEL GGNPLKNSGI ENGAFQGLKS LSYIRISDTN ITAIPQGLPT SLTEVHLDGN KITKVDAPSL KGLINLSKLG LSFNSITVME NGSLANVPHL RELHLDNNKL LRVPAGLAQH KYIQVVYLHN NNISAVGQND FCRAGHPSRK ASYSAVSLYG NPVRYWEIFP NTFRCVYVRS AIQLGNYKHH HHHH |
Decorin (DCN) is a small cellular or pericellular matrix proteoglycan which is closely related in structure to biglycan protein. Decorin is a secreted protein which binds to collagen and fibronectin in extracellular matrix. Decorin appears in different glycoforms, substituted with chondroitin sulfate or dermatan sulfate consistent with the original tissue. DCN contains one attached glycosaminoglycan chain. Decorin influences the rate of fibril formation. Decorin is capable of suppressing the growth of various tumor cell lines. DCN gene defects cause corneal dystrophy. The DCN gene is a candidate gene for Marfan syndrome.
DCN produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (17-354 a.a.) and fused to a 6 aa His Tag at C-terminus containing a total of 344 amino acids and having a molecular mass of 38.8kDa.DCN shows multiple bands between 40-57kDa on SDS-PAGE, reducing conditions and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | DCN: May affect the rate of fibrils formation. Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD). Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Secreted; Extracellular matrix; Secreted, signal peptide; Motility/polarity/chemotaxis Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region; collagen type VI Molecular Function:collagen binding; glycosaminoglycan binding; extracellular matrix binding; protein N-terminus binding Biological Process: peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan |
| NCBI Summary: | This gene encodes a member of the small leucine-rich proteoglycan (SLRP) family of proteins. The encoded preproprotein is proteolytically processed to generate a mature protein product, which is secreted into the extracellular space to regulate collagen fibril assembly. Homozygous knockout mice for this gene exhibit enhanced tumorigenesis in a liver cancer model, and defects in collagen fibrils, leading to weakened skin and tendons. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015] |
| UniProt Code: | P28654 |
| NCBI GenInfo Identifier: | 129952 |
| NCBI Gene ID: | 13179 |
| NCBI Accession: | P28654.1 |
| UniProt Related Accession: | P28654 |
| Molecular Weight: | 39,809 Da |
| NCBI Full Name: | Decorin |
| NCBI Synonym Full Names: | decorin |
| NCBI Official Symbol: | Dcn |
| NCBI Official Synonym Symbols: | DC; PG40; PGII; PGS2; mDcn; DSPG2; SLRR1B |
| NCBI Protein Information: | decorin; bone proteoglycan II |
| UniProt Protein Name: | Decorin |
| UniProt Synonym Protein Names: | Bone proteoglycan II; PG-S2; PG40 |
| Protein Family: | Decorin |
| UniProt Gene Name: | Dcn |
| UniProt Entry Name: | PGS2_MOUSE |
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