The Mouse DAT (Dopamine Transporter) CLIA Kit is specifically designed for the quantitative measurement of dopamine transporter levels in mouse serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.The dopamine transporter is a key protein involved in the regulation of dopamine levels in the brain, playing a crucial role in various neurological and psychiatric disorders such as Parkinson's disease, ADHD, and addiction.
By accurately measuring dopamine transporter levels, researchers can gain valuable insights into the mechanisms underlying these disorders and develop targeted therapies for their treatment.Overall, the Mouse DAT CLIA Kit from Assay Genie is a valuable tool for studying dopamine transporter function and its implications in neurological and psychiatric disorders, providing researchers with reliable data to advance their understanding of these conditions.
Product Name:
Mouse DAT (Dopamine Transporter) CLIA Kit
SKU:
MOES00230
Target:
Mouse DAT (Dopamine Transporter)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
1.88 pg/mL
Detection range:
3.13-200 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse DAT. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse DAT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse DAT, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse DAT. You can calculate the concentration of Mouse DAT in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-104
102-119
92-108
Average (%)
99
110
99
1:4
Range (%)
91-104
87-102
102-120
Average (%)
96
94
110
1:8
Range (%)
99-113
84-100
96-107
Average (%)
105
91
101
1:16
Range (%)
90-104
103-119
89-103
Average (%)
95
109
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-107
98
EDTA plasma (n=5)
100-118
108
Cell culture media (n=5)
91-105
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.83
21.79
99.68
9.1
20.23
106.91
Standard deviation
0.91
1.66
7.62
0.89
2.33
12.2
C V (%)
9.26
7.62
7.64
9.78
11.52
11.41
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse DAT concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse DAT in samples. No significant cross-reactivity or interference between Mouse DAT and analogues was observed.
