The Mouse Cytochrome P450 11B1 (CYP11B1) ELISA Kit is a highly sensitive and specific assay for the detection of CYP11B1 levels in mouse serum, plasma, and tissue lysates. This kit offers accurate and reproducible results, making it an excellent tool for a variety of research applications.CYP11B1 is a key enzyme involved in the biosynthesis of steroid hormones, particularly in the production of cortisol and aldosterone. Dysregulation of CYP11B1 activity has been implicated in various conditions such as adrenal disorders, hypertension, and metabolic syndrome.
Therefore, measuring CYP11B1 levels can provide valuable insights into these diseases and aid in the development of targeted therapeutic interventions.With its reliable performance and ease of use, the Mouse CYP11B1 ELISA Kit is a valuable asset for researchers investigating steroid hormone metabolism and related pathologies in mouse models.
Product Name:
Mouse Cytochrome P450 11B1 (CYP11B1) ELISA Kit
Product Code:
MODL00341
Size:
96 Assays
Target:
CYP11B1
Synonyms:
CPN1, CYP11B, FHI, CYPXIB1, S11BH, 11-Beta-Hydroxylase, Cytochrome P450 Family 11 Subfamily B Polypeptide 1, Steroid 11-beta-hydroxylase, Cytochrome P-450c11
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
