Mouse CYP2E1 (Cytochrome P450, family 2, subfamily E, polypeptide 1) CLIA Kit
The Mouse CYP2E1 (Cytochrome P450 Family 2 Subfamily E Polypeptide 1) CLIA Kit is specifically designed for the accurate detection of CYP2E1 levels in mouse samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.CYP2E1 is an important enzyme involved in the metabolism of various xenobiotics and endogenous compounds in the liver. Its expression and activity can be influenced by factors such as alcohol consumption, drug interactions, and exposure to environmental toxins.
Understanding the role of CYP2E1 is crucial for studying drug metabolism, toxicology, and liver diseases.With this CLIA kit, researchers can reliably measure CYP2E1 levels in mouse samples, providing valuable insights into drug metabolism, toxicology, and liver function. This kit is a valuable tool for studying the impact of CYP2E1 in disease pathogenesis and developing potential therapeutic interventions.
Product Name:
Mouse CYP2E1 (Cytochrome P450, family 2, subfamily E, polypeptide 1) CLIA Kit
SKU:
MOES00621
Target:
Mouse CYP2E1 (Cytochrome P450, family 2, subfamily E, polypeptide 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse CYP2E1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CYP2E1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CYP2E1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse CYP2E1. You can calculate the concentration of Mouse CYP2E1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-106
96-110
102-116
Average (%)
100
102
109
1:4
Range (%)
100-117
89-102
100-115
Average (%)
107
94
108
1:8
Range (%)
90-100
92-107
102-117
Average (%)
95
99
109
1:16
Range (%)
90-103
97-115
92-108
Average (%)
95
105
99
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-112
107
EDTA plasma (n=5)
88-100
95
Cell culture media (n=5)
99-114
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.86
3.63
16.2
1.77
3.3
15.97
Standard deviation
0.2
0.41
1.28
0.17
0.36
1.58
C V (%)
10.75
11.29
7.9
9.6
10.91
9.89
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse CYP2E1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse CYP2E1 in samples. No significant cross-reactivity or interference between Mouse CYP2E1 and analogues was observed.
