Mouse Cyclin Dependent Kinase Inhibitor 2A (CDKN2A) ELISA Kit

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SKU:
MODL00248
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q64364
Sensitivity:
0.108ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
P16,ARF,CDK4I,CMM2,INK4,INK4a,MLM,MTS1,TP16,P14,P14ARF,P16INK4a,P19,Melanoma P16,Cyclin-dependent kinase 4 inhibitor A,Multiple tumor suppressor 1
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
Frequently bought together:

Description

system_update_altDatasheet

Mouse Cyclin Dependent Kinase Inhibitor 2A (CDKN2A) ELISA Kit

The Mouse Cyclin-Dependent Kinase Inhibitor 2A (CDKN2A) ELISA Kit is specifically designed for the accurate and sensitive detection of mouse CDKN2A levels in serum, plasma, and cell culture supernatants. This kit offers high precision and reliability, ensuring consistent and reproducible results for various research applications.CDKN2A is a crucial protein that acts as a tumor suppressor by inhibiting cyclin-dependent kinases, hence regulating cell cycle progression and cell growth.

Dysregulation of CDKN2A has been implicated in various cancers and other diseases, making it a valuable biomarker for studying these conditions and potential therapeutic targets.Overall, the Mouse CDKN2A ELISA Kit provides researchers with a powerful tool for investigating the role of CDKN2A in disease pathogenesis and for developing novel strategies for diagnosis and treatment.

Product Name:Mouse Cyclin Dependent Kinase Inhibitor 2A (CDKN2A) ELISA Kit
Product Code:MODL00248
Size:96 Assays
Target:CDKN2A
Synonyms:P16, ARF, CDK4I, CMM2, INK4, INK4a, MLM, MTS1, TP16, P14, P14ARF, P16INK4a, P19, Melanoma P16, Cyclin-dependent kinase 4 inhibitor A, Multiple tumor suppressor 1
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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